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The graft vein specimens were cut on each time point.
Saphenous vein specimens from non-varicosed parts of vein were processed as above.
We collected blood and vein specimens from 159 consecutive patients undergoing varicose vein surgery, or autologous vein reconstruction for arterial occlusive disease as controls.
We studied saphenous vein specimens of patients undergoing surgical coronary revascularisation in 2007 and compared results with those of patients examined in 2003.
T1-weighted (T1W), T2*-weighted (T2*W), and proton density-weighted (PDW) high-resolution (spatial resolution 0.3 × 0.3 × 0.5 mm) MR imaging of labeled and control vein specimens was performed on a clinical 3 T MRI scanner (HDx, General Electric, Milwaukee, WI) equipped with 40 mT/m gradients (150 T/m/s slew rate).
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If the LI is between 2 and 4, we look for "contralateral suppression," meaning that the A C ratio in the lower adrenal vein specimen is lower than in the IVC, which serves as evidence that the lower side is not producing significant aldosterone.
Conclusion: In our in vitro closed-loop model, reproducible vessel wall changes were observed in all human vein graft specimens studied.
For DNA extraction from vein tissue specimens, a standard salting out procedure was used.
Blood and vein tissue specimens were kept on ice for a maximum of 6 h until storage at − 80 °C.
Vein tissue specimens were obtained from all patients during surgery by dissecting an appropriate piece of tissue (at least 1 cm) from affected or unaffected (controls) veins and transferred into a freezing vial.
Blood and vein tissue specimens were collected from 159 consecutive surgical patients, including 116 with primary varicose veins and 37 controls with arterial occlusive disease, at the Maria-Hilf-Krankenhaus in 54550 Daun, Germany, and at the Johanniter-Krankenhaus in 47228 Duisburg, Germany, during a time period from April 2011 to August 2013.
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