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Incubation media of control cells contained the same vehicle concentration of 0.1% (v/v).
As DMSO had been used regularly to solubilise substrates and compounds such as 7-ER, α-naphthoflavone and SKF-525A at a concentration of 0.1% v/v, the effect of increasing the DMSO vehicle concentration from 0.1%to0.2%2% upon EROD activity was assessed.
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In fact, such full-stop queues are rare events, while in most cases congestion waves propagate through the traffic stream, so that on a bridge there are periodically times of closely-spaced vehicle concentrations and times of flowing traffic, where vehicles are more distant.
Vehicle concentrations tested were therefore 0.1% albumin and 0.0789, 0.789 and 7.89 μM ethanol.
Based on previous results (Upham et al. 1996, 1998), 1-methylanthracene as well as PFOA were used as positive controls of inhibition of GJIC, whereas acetonitrile at vehicle concentrations was used as a negative control.
Ambient NOx measurements are likely to be a poor reflection of roadway or in-cabin vehicle concentrations owing to the rapid transformation of emissions with distance from the roadway.
Moreover, in-vehicle concentration levels, while driving along major avenues and in the heavy-trafficked centre of Athens, were measured (mean route duration: 45 min).
In the major tunnels, transit-average in-vehicle NO2 concentrations were lower than outside-vehicle concentrations for all vehicles with cabin air recirculation either on or off.
There is a lack of knowledge regarding in-vehicle concentrations of nitrogen dioxide (NO2) during transit through road tunnels in urban environments.
Finally, the highest in-vehicle concentrations (148.0 · 103 173.0 · 103 particles/cm3) were measured in central commercial areas of Athens during, on average, 55 min drives.
In addition, while we have included the effects of emissions on ambient concentrations, we have not captured the effects on in-vehicle concentrations and personal exposures.
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