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In this way, 10 mice received an approximate local dose of simvastatin of 0.1 mg per kg per day for the duration of the experiment and 10 mice received the vehicle compound.
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The effect of vehicle, compounds, and standard drug on blood glucose level was determined in the animals at 0, 4, 8, and 24 h after daily oral administration.
Human SHSY5Y-APP neuroblastoma cells were treated for 24 h with either vehicle (DMSO), compound, 49 or compound 46.
Compared to vehicle treatment, compound 11 had no significant effect on the plasma aldosterone concentrations in either lean (vehicle: 149 ± 28 pg/mL; compound 11: 186 ± 28 pg/mL) or obese (vehicle: 145 ± 26 pg/mL; compound 11: 184 ± 31 pg/mL) SHR-cp.
Compared to the vehicle treatment, compound C markedly reduced the activation of AMPK.
Animals received 0.5 μl/h of vehicle or compound for 1 week.
Occasionally, minor changes in calcium were observed after addition of either vehicle or compound.
Fifteen minutes prior to seizure induction, mice were treated with the vehicle or compound B (n = 12 per group).
Animals were injected with either vehicle or compound AST3-88 after completion of overnight fasting with food being returned 90 min after treatment.
The renin-angiotensin-aldosterone system was assessed by measuring circulating renin and aldosterone concentrations before and after chronic vehicle or compound 11 administration.
Oral glucose tolerance testing was conducted before and 4 weeks after vehicle or compound 11 administration in lean and obese SHR-cp.
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