Sentence examples for vegetative samples from inspiring English sources

Vegetative samples were grown in YPD (1% yeast extract, 2% peptone, 2% dextrose) to an OD600 of 0.6 0.8.

Four competitive hybridizations, including two dye-swaps, were performed between cDNA of the sexual and vegetative samples.

The amount of glucose released from the (1,3 1,4 -β-glucan in the vegetative samples was quantified based on the colorimetric assay using GOPOD, Megazyme International Ireland Ltd., according to Ermawar et al. [ 61,3 1,4 -β-glucan

GeNorm application was used for evaluation of the best reference genes, and according to that, the least stable genes, with the highest M values were tubulin and succinyl-CoA ligase and the most stable ones, with the lowest M values were elongation factor 1 alpha and ubiquitin conjugating enzyme, when both reproductive and vegetative samples were tested.

Harvesting was done analogous to the vegetative samples at nine and ten hours post-illumination, during which cell-pairing (and thus meiosis) was observed, and at 19, 20 and 21 h, when auxosporulation occurs and thus the meiotic phase is passed.

The vegetative category contains all genes that exhibited a statistically significant higher expression in the vegetative tissue compared to the sexual, and the constitutive category consisted of all genes that did not exhibit differential expression between the sexual and vegetative samples.

For the vegetative sample, total RNA from mycelia of each strain was pooled in equal amounts.

In addition, a separate library was constructed from the vegetative sample.

A pooled sample of RNA or mycelia of the two strains was used as the vegetative sample in subsequent microarray and EST-analyses, respectively (see below).

However, while we were able to detect tasiRNAs in mature pollen by RT-PCR, their precursor TAS transcripts proved no longer detectable - although these were readily amplified from a control vegetative sample (see Additional file 1: Figure S4).

Each reaction was performed in duplicate, and the average Ct value from the uninduced vegetative cells (T = 0) was subtracted from the average Ct values at each time point (T = 0.5, 1, 2, and 4 hours after induction) in order to present the expression relative to the uninduced vegetative sample (T = 0).