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Thus, the SDS-PAGE pattern of the analyzed vegetal extract presented a major protein band, identified as DAO, whose growing intensity was in function of the concentration of the loaded vegetal extract (Fig. 1a).
These results suggest a possible protection of DAO afforded by the other proteins present in the vegetal extract or a possible presence of some protease inhibitors in the vegetal extract able to partially protect the DAO against pancreatin proteolysis.
Control was prepared by adding ethanol instead of vegetal extract, and a blank without liposomes but methanol was also analyzed.
In this set of experiments the cell viability was not modified after 1 h incubation with both vegetal extract and the pure flavonoid.
The elucidation of these aspects, explaining better stability to proteolysis in the case of the vegetal extract, will be the object of a further investigation.
The same migration patterns of molecular weight standards and of vegetal extract were obtained for either gels containing or not entrapped peroxidase after Coomassie coloration (data not shown).
Similar(27)
TPA – ear model is useful for the screening of new topical anti-inflammatory compounds and vegetal extracts.
Mixed vegetal extracts are interesting target of new products as nutraceuticals, superior ingredients for the design of functional food, singular ingredients for cosmetics, etc.
DPPH assay has confirmed high antioxidant activity of both vegetal extracts, even stronger than that of references, rutin and gallic acid phenolics (IC50 ranging from 2.66 to 5.08 μg / mL).
In vivo, pharmacological studies have indicated good dermal tolerance and healthy, supple and reepitelized tegument after being treated with both vegetal extracts, which has revealed their potential use as antioxidant and healing ingredients for (topic) biopharmaceuticals.
The iron-gallic dyes, on the basis of the ancient recipes, were produced by mixing vegetal extracts (tannins obtained by gall-nuts) with inorganic salts (mainly vitriols, nominally iron sulphates with other metal sulphates such manganese, copper, zinc, lead and others).
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