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On this base, the investigations on non-viral gene vectors with the ability to overcome physiological barriers are increasing.
The design of non-viral gene vectors with the ability to overcome delivery obstacles is currently under extensive investigation.
Efforts aimed at engineering vectors with the ability to evade the immune response include identification of residues on the virus capsid important for these interactions and changing them.
Poly(diethylaminoethylmethacrylate) (PDEAEM) and Pluronic F127 based pentablock copolymer vectors with the ability to transfect cancer cells selectively over normal cells in in vitro cultures were developed, as described in a previous report.
Because of their bilipidic structure, they are flexible vectors with the ability to carry select nucleic acids (miRNA, siRNA, and mRNA), proteins, and active chemical drugs across biological barriers [ 74, 78, 79].
The development of new vaccines requires a clear understanding of the nature of both innate and acquired immunity in the lung and the role that these two sets of mechanisms play in the protective immune response against M. tuberculosis, as well as the construction of delivery vectors with the ability to elicit an optimal protective response that controls the pathogen.
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Moreover, one adaptive weight vector with the ability to block the desired signal in the auxiliary array can be obtained simultaneously, which effectively avoids the signal of interest cancellation consequently.
A new paradigm of therapeutics, named multistage vectors, has emerged, with the ability to preferentially target tumors while protecting and delivering payload to the site of action.
The ease of transduction with viral vectors and the ability of these cells to cross the endothelial wall in the presence of inflammation, as in the case of muscular dystrophy, makes them very interesting therapeutic candidates for systemic delivery 80.
Compared with non-integrating vectors, the ability of lentiviral vectors to integrate is an important advantage when targeting muscle diseases, such as Duchenne muscular dystrophy (DMD) [4], [5], [6], [7], [8], [9], [10].
This tropism resulted in gene transfer vectors based on BAAV having a unique tropism compared with current AAV vectors and the ability to transduce cells in the inner ear [8] [10].
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