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To address the function of KLF13 in the early stage of porcine adipocyte differentiation, we established three different small interference RNA (siRNA) sequences against KLF13 and constructed KLF13 overexpression vector (Materials and methods).

We cloned the same engineered and native FtsZ, NS5B and P38α genes into a CFS expression vector (Materials and Methods) ensuring that the full length proteins had identical amino acid sequences.

The issue of systemic spill over of vector material into the cardiac atria and thereafter into the systemic circulation would need to be evaluated by sampling local and distant organs.

As expected, cybrids transiently transfected with mock vectors (see Materials and Methods) retained the pathological phenotype (supplementary Fig S1).

Nevertheless, the final classifier used four features because of our strategy of simplifying the model by also minimizing the number of support vectors (see "Materials and Methods").

We generated a set of 11 pigP vectors (supplementary Materials and Methods and Fig. S1A) that contained either two copies of StrepTagII and a 3×FLAG epitope fused in frame to the Venus-YFP-coding region in all three reading frames (FSVS vectors), or identical constructs with only two copies of StrepTagII (SVS vectors; supplementary material Fig. S1B).

FLcDNAs were expressed in E. coli using one of several cloning vectors (see Materials and Methods), and recombinant VvTPS proteins were functionally characterized via purified protein or in vivo recombinant E. coli assays with each of the following potential substrates, geranyl diphosphate (C10, GPP), (E, E -farnesyl diphosphatE -farnesyl), andiphosphate-geranylgeranyl diphosphate (C15, GGPP).

Interference with Eva1 was performed by infection with lentiviral vectors (see material and methods for details).

Subsequently, we expressed HPV-16 E7 in HaCaT keratinocytes via the pLXSN retroviral vector (see Materials and Methods).

Two BP reactions were set between the expression clone and either an attP3/P5 (Fig. 7A) or an attP1/P2 cassette (Fig. 7B) purified from the corresponding donor vector (see materials and methods).

Vectorial additions were then performed for laterals from each cell vertex on the compound line using parallelogram method to create collections of new vectors (supplementary material Fig. S4D).