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The cDNA sequences were cloned into pFASTBACAHTa expression vectors, expressed in insect cells and purified as described [10].
bH1, bH1-81 and bH1-44 antibodies were isolated from phage displayed antibody libraries and cloned into Fab and IgG expression vectors, expressed in E. Coli and 293 cells respectively, and purified by protein A affinity chromatography as described [22].
These vectors expressed the target mRNAs fused to EGFP reporter genes.
Cells transduced with hybrid human/porcine fVIII encoding vectors expressed fVIII at levels 6- to 100-fold greater than cells transduced with vectors encoding human fVIII.
All the NDV vectors expressed gp160 protein in infected cells.
Cells transfected with SIVmac239Delta3DeltaLTR-vMIP-II SIVmac239Delta3DeltaLTR-vMIP-II SIVmac239Delta3DeltaLTR-vMIP-II4 cells and suppressed SIvectors infexpressed
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The reduction in vimentin expression, after transfection with vectors expressing TAp63, was also confirmed by immunofluorescence.
(3) Viral and plasmid vectors expressing therapeutic genes.
Lentiviral vectors expressing METTL3 in piPSCs was purchased from Hanbio (Shanghai, China).
E14-Cas9 mESCs were infected with lentiviral vectors expressing Cdx2-sgRNA and Gsk3α-sgRNA.
For the T (5:7) -E14 cells labeling, cells were infected with lentiviral vectors expressing GFP.
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