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The backbone vector without a gene insert was rescued as a negative control vector.
For all the in vivo and in vitro experiments, the vector without a gene inserted in the MCS was used as control (control vector).
In contrast, the cells with tdTomato sgRNA alone, Cas9 alone or empty sgRNA expression vector without targeting sequence revealed no visible difference from parental tdTomato knock-in cells.
A numerical integration of these distributions permits an accurate calculation of the Burgers vector without constructing a Burgers circuit.
The frequency at another site (BF10) was almost two-fold greater in comparison to the vector without homologous arms.
We showed that these four expression cassettes can be combined in a single lentiviral vector without causing recombination.
The thermo-chemical database is tabulated with respect to the thermodynamic state vector without any reduction in the number of state variables.
A region of Erh1 (residues 1 104) was amplified by PCR from the S. pombe genome and cloned into a modified pET28a (Novagen) vector without a thrombin protease cleavage site (p28a).
A transfection efficiency of > 45% is reported which is similar to what is achieved with polyethyleneimine (PEI), a non-viral gold standard vector, without causing cytotoxic side effects.
method to introduce multiple copies of provector within vector-producing cell lines (VPCLs), resulting in high-titer vector without the generation of RCR.
Cloning of the cPPT/CTS fragment in front of the 3′-LTR resulted as well in a vector with higher transduction efficiency than a vector without central flap.
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CEO of Professional Science Editing for Scientists @ prosciediting.com