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v m is the mixed speed vector, which was the tip speed after assistance.
DWT was used to extract the energy spectrum feature vector, which was regarded as ANFIS input.
Target genes were cloned into a transfer vector which was used to manipulate the viral genome.
VHH DNAs were digested with NotI and NcoI and then inserted into phage M13 vector which was pre-digested with NotI and NcoI.
Two candidate genes, xylanase (xynA) and glucanase (bglS) from B. subtilis strain 168, which were engineered with independent Shine Dalgarno (SD) sequences, were cloned in tandem into a transfer vector, which was then transformed into B. subtilis strain 1A304 (φ105MU331).
The amplified fragment was cloned into a pcDNA3.0 vector, which was termed pcDNA3-29a.
Therefore the highest expression and stability was achieved with the pMV306hsp integrating vector, which was selected for further studies.
Replication incompetent retroviral vector which was collected from PG13.VEGF cells was used for transfection of F3 human NSCs.
The targeting vector, which was constructed by ET recombination [52] with a 12.7kb genomic sequence, was introduced into 129Sv/R1 ES cells [34] by electroporation.
Each TOPO clone was sequenced in both directions and the inserts subcloned into the pGEMHE vector which was subsequently sequenced in both directions.
Plasmid constructs that expressed SGIV-encoded miRNAs were generated based on the pLL3.7 modification vector, which was the same as that used by Xia et al. [43].
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