Sentence examples for vector which contained from inspiring English sources

For functional complementation of rel2 mutant, the full length of WT cDNA of REL2 was cloned into the binary p35S-1301 vector, which contained a hygromycin resistance marker.

The pSIR-Con vector, which contained a scrambled shRNA sequence, was used alongside as negative control.

The zebrafish atoh8 cDNA was cloned into pGEM-T easy vector, which contained T7 and SP6 promoters.

First, we generated CD4+ C8166 T cell lines expressing either R88-A3G or HA-A3G fusion proteins by transducing C8166 T cells with a VSV-G pseudotyped lentiviral vector, which contained HA-A3G or R88-A3G trassgene, as described in Materials and Methods.

An empty vector (which contained five Ts after the U6 promoter) vector was used as a negative control.

For the generation of Sec7 deficient cells (sec7 - ) we used a gene replacement vector which contained nucleotides 1 to 441 and 1981 to 2469 of the cDNA.

shRNA constructs are in the pLKO.1 vector, which contains the gene for puromycin resistance.

The time stamps are combined with a position estimation vector which contains the approximate location of each of the sensor nodes.

The synj and mnb-F transgene constructs were generated by subcloning the coding regions of synj or mnb-F into the pINDY6 vector, which contains the HA tag, and transgenic flies were generated by standard transformation method.

The IgG expression cassette vectors are based on the backbone vector which contains two back to back polyhedron and p10 promoters.

In Equation 7, Θ = [θ1, θ2,…, θ z ] T is a column vector which contains M nonzero coefficients.