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In (B) and (C), A375 cells stably expressing STAT3C or empty vector were treated with indicated concentrations of SLE for 48 h.
A375 cells stably transfected with STAT3C or vector were treated with SLE (50 μg/mL) or vehicle control for 24 h.
There was no such interaction even when cells with the basic/empty vector were treated with HU.
(E) CGNs transfected with GFP vector plus wild type MCU, MCUD260A mutant, MCUE263A mutant or empty vector were treated with 70 μmol/L H2O2 for 24 h.
(A) Cells of the Atg31∆ strain expressing wild-type (WT) Atg31, the Atg31-Ser174 mutant (S174A), or a control vector were treated by nitrogen starvation (SD-N), rapamycin or amino acid starvation (SD-AA) and assessed for autophagy activity.
Cultures of cells containing empty vector were treated identically as controls to monitor basal prion stability.
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When the linearized vector is treated with T4 DNA polymerase, in the presence of the absent base, long single-stranded overhangs are generated that are suitable for cloning.
All the features extracted were combined into a vector and the vector was treated as distinguishing features for splicing detection.
In previous PST studies [1, 2], the surface normal vector and the error vector are treated as two entities and are solved independently.
To obtain in-frame fusions, the resulting vectors were cut with BglII and subsequently 1 µg of cut vector was treated with 5 U mung bean nuclease (New England Biolabs) for 1 hr at 30°C to remove overhangs and ligated.
The HA encoding gene in the pCI vector was treated with Xho I, Klenow polymerase fragment, then subsequently digested with Sal I, and cloned into the pFastBac plasmid, a baculovirus (BV) transfer vector (Invitrogen).
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