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Co-injections of zTERT-MO1 or -MO2 together with either the GFP-zTERT or control GFP vector were then performed.

Thiamphenicol resistant transconjugants of C. botulinum containing the ClosTron vector were then plated onto agar supplemented with erythromycin for selection of intron integrants, since the erythromycin resistance gene is restored upon integration of the group II intron [20], [21].

The isolated cDNA and Sma-I linearized pGADT7-Rec2 vector and bait vector were then transformed into yeast strain Y187, and the transformants were selected on SD/-His/-Leu/-Trp SD/-His/-Leu/-Trp SD/-His/-Leu/-Trp SD/-His/-Leu/-Trp

The PCR products and vector were then digested with these two enzymes and ligated to screen the promoter-luc2 constructs.

Transformants containing an integrated vector were then treated with 5-FOA to select for excision of the plasmid sequences leaving the EI expression cassette in the chromosome.

Cells stably expressing C35 or control vector were then isolated, and the expression of C35 was confirmed by western blotting using an anti-Myc antibody (Santa Cruz).

Vector is then collected from.

This vector is then pushed to the coordination service.

The feature vector is then generated based on these features.

A perfusion vector is then calculated using Eq. 2.

The normalized HOG vector is then calculated as follows [12].