Linearized and ligated vector were separated on a 1% agarose gel.
The reactions were incubated for 60 min at 37°C and then stopped by the addition of 15 µl of loading buffer followed by heating to 75°C for 10 min. The in vitro generated transcripts from the linear templates, which contained the cloned PhilD or PhilD*, the vector promoter (Pν), and the supercoiled pUA1111 vector, were separated in 6% dPAGE, visualized, and quantified as described [65].
The attB and attP sites on this vector are separated by only 6 bp.
The alternative algorithm is binning where the coverage vector is separated into equal intervals and the average value for each interval is calculated.
HNP-1, HBD-2 or RTD-1 were incubated with the pseudotyped vectors for one hour on ice and then defensin and lentiviral vectors were separated on a G-25 Sephadex column as described above.
Both 2D patterns obtained perpendicular to the flow direction were anisotropic, and two sets of spots at scattering vectors were separated azimuthally by 180°, as expected for an oriented cylindrical morphology (see Figure S2 in the Supporting Information).
Assuming that the input frames contain two different areas, including background (non-skin) and foreground (skin) areas and their motion vectors are separated by threshold t, the co-occurrence matrix is divided into four quadrants, which represent background-to-background (BB), background-to-foreground (BF), foreground-to-background (FB), and foreground-to-foreground (FF) regions.
In Figure 2, the contributions of each order (n = 1, 2, 3,…) of the dyadic Green's functions, which are series solutions in terms of spherical wave vectors, are separated individually from the radiative and nonradiative powers: the dipole mode (n = 1), quadrupole mode (n = 2), sextupole mode (n = 3), octupole mode (n = 4), etc.
As a result, two lights are discriminable if their vectors are separated by more than the noise.
Insert fragments to be used as probes for genomic DNA blots were separated from vector sequences on 0.7% agarose gels and extracted by electroelution [ 38].
After quenching the crosslinking reaction, the hix recombination sites were separated from vector sequences using EcoRI, the EcoR1 ends were P-labeled, and the products were analyzed by SDS-PAGE (Dhar et al., 2009a).
