Sentence examples for vector were seeded from inspiring English sources

For DNA transfection, 1×106 CHO cells containing the MI-HAC vector were seeded into wells of 6-well tissue culture plates 24 h before transfection.

For DNA transfection to construct the MI-HAC vector, 1×106 HPRT-deficient CHO cells containing the HAC vector were seeded in wells of 6-well tissue culture plates 24 h before transfection.

Subsequently 2×105 66c14 cells transfected with versican G3 or control vector were seeded onto 6-well culture dishes in DMEM medium with varying amounts of FBS (2.5, 5, and 10%) for 3 h.

Briefly, the MGC803 cells transfected with OCT1 vector, OCT1 vector+synbindin siRNA and control vector were seeded onto 96-well plates at 2500cells/well.

Brca1-deleted cells with an empty RMCE vector were seeded at 1250 cells per well to compensate for their proliferation defect.

An amount of 104 cells of parent CNE2 cells, CNE2- TFPI-2 and CNE2-empty vector were seeded into 6-well plates.

Cell lines transfected with synthesized oligonucleotides or vectors were seeded in 12-well plates (100 cells per well) and incubated for 7 10 days to allow colony growth.

Immortalized 3000 cells of 100166/1T cells transduced with the retroviral control or UBE2T vectors were seeded in wells of a 96-well plate.

Cells infected with either miR-205 expression or control lentiviral vectors were seeded into inserts at 2 × 10 per insert in serum-free medium and then transferred to wells filled with the culture medium containing 10% FBS as a chemoattractant.

Stable B1041-1 celinesnes containing PML I, PMLIb or vector alone were seeded at ∼5×106 cells per well in 6-well clusters and mock infected, or exposed to HSV-1 strain F or the recombinant HSV-1 ICP0 null mutant virus R7910 diluted in mixture 199 (Gibco) supplemented with 1% calf serum.

To assess cell proliferation, 500 SCC4-HOXandand SCC4-empty vector cells were seeded in 96-well culture plates.