Sentence examples for vector were quantified from inspiring English sources

In addition, infectious titers of the LV-GFP vector were quantified by infection of 293T cells, in which the R0.8 promoter is active, followed by flow cytometry on a FACSCalibur (Becton Dickinson, Franklin Lakes, NJ, USA).

The proteins (Pro, P10, P8, RdRp, VPg and CP) expressed from pGAD T7 vector were quantified using HA polyclonal antibody as primary antibody, where as MP and the deletion mutants of MP expressed from pGBKT7 were quantified using cMyc monoclonal antibodies.

The vector containing the insert was purified from the positive colonies using the Qiagen Plasmid Midi purification kit (Qiagen Inc., Valencia, CA, USA) and the concentration of the vector was quantified by a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies. Thermoscientifics. Wilmington. USA).

Transfection efficiency and cellular internalization of the vectors were quantified in the presence of a panel of inhibitors of various endocytic pathways.

Vectors were quantified using the forward primer 5'-GGCGGAGTTGTTACGACAT-3' and the reverse primer 5'-GGGACTTTCCTACTTGGCA-3' specific for the CMV promoter sequence.

To verify that inducing expression of the lysozyme variants in the chemostat cultures was taking place and producing the expected consequences, lysozyme transcripts originating from the induction vectors were quantified using qRT-PCR and secreted lysozyme activity determined using an enzymatic assay (Fig.  2a, b).

For this purpose, the uncertainty in the observed difference of the sample mean vectors is quantified by defining a confidence region which contains the true difference of the population mean vectors with a prescribed probability.

To provide a comprehensive evaluation, in our study the differences between the hyper-spectral feature vectors are quantified using classical metrics that were widely used in the development of hyper-spectral classification schemes.

RNA content of lentiviral vectors was quantified with a one-step RT-qPCR that allows reverse transcription (RT) and amplification to take place in the same reaction tube.

Vector genomes (vg) were quantified by dot blot.

Vector copies per diploid genome (vector copy number, VCN) were quantified by quantitative PCR (qPCR) starting from 100 ng of template gDNA using primers (HIV sense: 5′-TACTGACGCTCTCGCACC-3′; HIV antisense: 5′-TCTCGACGCAGGACTCG-3′) and a probe (FAM 5′-ATCTCTCTCCTTCTAGCCTC-3′) against the primer binding site region of LVs.