Sentence examples for vector were plated from inspiring English sources

MDCK cells transfected with the gene trap vector were plated in nine 100 mm dishes (approximately 3.3×106 cells per dish) in Leibovitz's L-15 medium.

In order to determine the kinetics of BRCA1-induced plasmid loss and subsequent lethality, we performed a "pullback" experiment in which WT cells containing the BRCA1 plasmid or empty vector were plated within GAL-URA or GAL+URA medium to induce BRCA1 expression.

Cells stably transfected with pEGFP-N1 MT1G or empty vector were plated in 96-well plates and cultured with 0.5% FBS.

For miRNA profiling, HEK-293 cells transduced with either FOXO1-AAA-ER or an empty vector were plated on two 10 cm plates, 4.0×10 cells per plate.

Stable cell lines transduced with Prx3, Trx2 and PGC-1α containing lentiviral vector or empty pRRL vector were plated in 96-well plates (Greiner Bio-One, Frickenhausen, Germany) and grown until confluent.

For colorimetric focus-formation assay analysis, MCF-7 cells stably transfected with pcDNA3.1-PinX1 and empty vector were plated in 6-well plates at a density of 10,000 cells per well, fixed by 100% of cold EtOH after 2 weeks of culture, and dyed by 5% crystal violate (Amresco, USA) in 30% of EtOH.

200  μL MCF-7 and LCC2 cells suspension with the recombinant lentiviral vector was plated into the upper chambers, and the medium with 10% FBS was piped into the lower Transwell chamber.

Yeast cells with co-transformed pDBLeu- and pPC86-derived vectors were plated and incubated on synthetic medium lacking leucine, tryptophan (DOB-Leu-Trp) and the selective medium DOB-Leu-Trp-His (supplemented with 50 mM of 3-amino-1,2,4-triazole 3-amino-1,2,4-triazole 3-amino-1,2,4-triazole 3-amino-1,2,4-triazolee His reporter gene attivity.

Expression vectors were plated, and single colonies were selected for mini-prep.

For this test, AH109 yeast cells transformed with DB vectors were plated on nutritionally selective medium deficient in tryptophan, adenine, and histidine.

293 cells overexpressing SALL4B or vector control were plated in a 96-well plate and treated with various concentrations of doxorubicin or daunorubicin.