For the infectivity analysis of biotinylated Ad5.CMV-LacZ, dilutions of biotinylated viral vectors were placed over monolayers of D-17 target cells on a 96-well cell culture plate (8 × 103 cells per well), followed by the incubation of cells at 37°C for 48 h.
After the above operations, the two resulting vectors are placed in the new population.
Perpendicular to this midline, normal vectors are placed connecting corresponding sectors in the end-diastolic and end-systolic frame.
One may question the usefulness of planthopper resistance QTL, because the RHBV resistance QTL seems to act genetically independently and is sufficient to bring high levels of resistance to the virus under controlled greenhouse or field conditions in which viruliferous vectors are placed on the plants to be tested.
Although the two genes were coexpressed on one expression vector, they were placed under two separate T7 lac promoters so that the expression of one gene would not affect that of the other.
For colony formation assay, a total of 500 pCDNA-MEG3 and empty vector cells were placed in a fresh six-well plate and maintained in media containing 10% FBS, replacing the medium every 4 days.
In these vectors, miRNAs were placed downstream of the LTR promoter, which is followed either by a SV40-GFP cassette (for all in vivo experiments), a PGK-Puro-IRES-GFP cassette, or a SV40-CD4 cassette (for in vitro experiments) (Hemann et al., 2005).
To determine if the 5' RhPV IRES element could be used to express more than one heterologous sequence from a dsSINV vector, RhPV 5' IRES sequences were placed between genes for two different fluorescent marker proteins in the dsSINV, TE/3'2J/mcs.
lin-35 ; slr-2 ; kuEx119 larvaevae were placed on vector-control or suppressor RNAi feeding plates and allowed to develop into adults.
The unique TCR α and β genes, specific for viral antigen, were placed into this vector separated with a 2A self-cleaving peptide (or "skip" peptide) where the two chains are expressed together with high efficiency [29](Figure 1A).
