Sentence examples for vector were mixed from inspiring English sources

The four pENTR vectors and a pDEST vector were mixed in the presence of LR clonase enzyme to generate the multi-integrase platform plasmid (Fig. 1B).

Around 20 ng of each entry clone and 80 ng of destination vector were mixed with 2 µl of LR clonase II Plus enzyme mix, and made up to 10 µl with TE buffer.

When cells reached about 70% confluence 95 μg of pVSVG, 68 μg of pREV, 176 μg pMDL, and 270 μg transgene containing lentiviral vector were mixed.

For ICAM-5 rescue experiments, plasmid p-EGFP-N1 and ICAM-5 constructs (PEF-BOS-ICAM-5, PEF-BOS-ICAM-5 Δcp or the empty vector) were mixed at 1 10 ratio and co-transfected into neurons.

Luciferase reporter plasmids, pRL-TK plasmid, pcDNA3.1-LANA, pcDNA3.1-LANA-C, or pcDNA3.1 empty vector were mixed and transfected into cells.

For each dish, 10 µg of the lentiviral vector were mixed with 3.5 µg of the VSV- G envelope plasmid and 6.5 µg of the packaging plasmid (pMD2.VSVG and pCMVΔR8.74, respectively [29]).

For bottom-up in vitro SCRaMbLE, 200 ng acceptor vector was mixed with the donor fragments pool (1000 ng in total) in a reaction volume of 50 μl with 1 μl of high concentration Cre recombinase (NEB, M0298M).

In the binomial crossover, the target vector is mixed with the mutated vector, using the following scheme, to yield the trial vector (u_i^G ).

In co-transfection experiments, pCMV-Sp1 or pSG5-Ap1 expression vector was mixed with the luciferase vectors at 1 4 concentration ratio.

Then, 20 µg pGCL vector was mixed with 15 µg pHelper1.0 vector (gag/pol component) and 10 µg pHelper2.0 vector (VSVG component), packed and transfected into 293T cells.

100 μl sterile water containing 20 μg/ml vector was mixed with 4 μl FuGENE HD transfection agent (Roche, Basel, Switzerland).