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The correctly fused pCAMBIA35S::OsHox32-GFP vector and empty vector were introduced into tobacco leaves (Nicotiana tabacum) to produce transient expression via infiltration of an Agrobacterium tumefaciens strain.
Then, the resultant plasmid and the empty vector were introduced into Agrobacterium tumefaciens EHA105 and then used to infect calli of WT plants according to a published method (Hiei et al. 1994).
To analyze tobacco epidermal cells, the fusion vector and empty vector were introduced into the Agrobacterium tumefaciens strain and injected into tobacco (Nicotiana tabacum) leaves by infiltration of the Agrobacterium tumefaciens strain.
pESC-Leu2d::yNhaNT2, a similarly constructed control plasmids with yNhANT2, or the empty vector were introduced into the triple AAC knock-out yeast (URA3-AAC2) (Fig. 3).
Four µg of an I-SceI expression vector pCMV(3_NLS) HA-I-SceI or an empty vector were introduced into 3.5×105 cells by electroporation.
pCSGW K-ras V12 or empty vector were introduced with packaging plasmid pCMV-dR8.91 and envelope plasmid pMD2.G into HEK293T cells by transfection with the Fugene6 regent (Roche, Indianapolis, IN).
Similar(45)
A so called meter vector is introduced.
So new variable r(t), rotated vector, is introduced.
In [16, 17], Lie brackets, the parameter map, and the velocity vector are introduced.
The engineered vector was introduced into Agrobacterium tumefaciens, strain EHA105, by electroporation.
A crack deflection criterion based on the J-integral vector is introduced and implemented into a crack growth model.
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