Sentence examples for vector were harvested from inspiring English sources

Briefly, cells transfected with pcDNA3.1 or pcDNA3.1-FBLN1 vector were harvested and washed twice with PBS.

SPC-A1 and A549cells transfected with pCDNA-MEG3 and empty vector were harvested 48 hours after transfection by trypsinization.

A549 cells stably transfected with pCDNA-SPRY4-IT1 or the empty vector were harvested, washed with phosphate-buffered saline, and resuspended at 2 × 10 cells/ml.

Briefly, Agrobacterium tumefaciens cells, containing either the overexpression or silencing vector, were harvested by centrifugation at 5,000 rpm for 10 min and resuspended in liquid NN medium, containing 18.5 μg.mL-1 maltose to a final OD600 of 0.8.

SCG7901 cells transfected with pCDNA3.1-GAS5 or empty vector were harvested from six-well cell culture plates, washed with PBS, and resuspended at a concentration of 1 × 10 cells/mL.

Briefly, COS1 cells transfected with the p3.1M- DLK1-HA vector were harvested in the Phosphate Buffered Saline (GIBCO) supplied with 1% Igepal CA-630 and the complete protease inhibitor cocktail.

An overnight-grown Agrobacterium tumefaciens strain GV3101 carrying a CaMV 35S::ZmF3′H1 construct cloned into a pSR3000 vector was harvested by centrifugation and suspended in 200-ml solution containing 5% sucrose and 0.002% Silwet l-77 (Lehle Seeds, Round Rock, TX).

Briefly, subconfluent human embryonic kidney 293T cells (ATCC) were cotransfected with 20 µg of a lentiviral vector LV-TH, 15 µg of pCMV-ΔR8.91, and 5 µg of pMD2G-VSVG vectors, After 16 h, the medium was changed, and recombinant lentivirus vectors were harvested 24 h later [46].

At 24 h after transfection, lentiviruses expressing miR-34c (lenti-miR-34c) or negative control empty lenti-vector (lenti-vector) were harvested and used to infect SUNE-1 cells, and stably transfected cells were selected using puromycin and validated by quantitative RT-PCR.

After 16 h medium was changed, and recombinant lentivirus vectors were harvested 48 h later.

After six hours, medium was changed and recombinant lentivirus vectors were harvested 24 hours later.