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MVECs transduced with DN or WT calpain-I or empty vector were grown to confluence in full medium.
Genes of interest cloned in L4440 vector were grown from standard C. elegans libraries and verified by sequencing.
HT115 bacteria transformed with L4440 with gene of interest or empty L4440 vector were grown in standard LB media with ampicillin (50 µg/ml) overnight at 37°C.
293T cells expressing FLAG-tagged SALL4B or an empty vector were grown to confluence on culture dishes in DMEM containing 10% FBS.
ynd1Δ cells transformed with wild-type (WT) Ynd1, an empty vector or each of the mutants, as well as E4orf4 or an empty vector, were grown overnight in liquid medium containing glucose as a carbon source.
Stable cell-lines, designated OKF-Δ1 (OKF cells containing the pESuper vector with siRNA1), OKF-Δ2 (containing pESuper with siRNA2), and OKF control cells (empty pESuper vector), were grown until confluence.
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A. rhizogenes without the binary vector was grown in YEP medium lacking antibiotics.
A control strain containing the empty vector was grown with continuous heat-shock or without temperature-induction.
A 5 mL starter culture of C41 cells transformed with each UPPS encoding vector was grown overnight.
A. tumefaciens strain EHA105 harboring the Fu39-8 Ub:: LUC vector was grown at 28°C in LB medium supplemented with appropriate antibiotics to stationary phase.
The A. tumefaciens strain LBA4404 harboring the pOEB5 vector was grown overnight at 28°C with shaking (150 rpm) in Luria-Bertani liquid medium containing 50 mg/L kanamycin.
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