Sentence examples for vector were diluted from inspiring English sources

Primary AML blasts samples transduced with control or JMJD3-overexpressed vector were diluted and seeded at 50,000 per 35-mm dishes in methylcellulose medium (MethoCult™ H4435, Stem Cell Technologies).

Briefly, 300 ng of the reporter vector and 300 ng of the control vector were diluted in OPTI-MEM media (GIBCO) containing 15, 30, 60 or 100 nM of the synthetic mature miRNA of interest in a final volume of 100 µl.

Overnight cultures of the Y. pestis ΔhmsTΔy3730 ΔhmsP strain transformed with different recombinant plasmids or with the empty plasmid vector were diluted 1∶30 into 40 ml of LB medium supplemented with 100 mg/l carbenicillin and grown at 28°C for 8 to10 h with shaking at 250 rpm.

Therefore, when the ROD/B vector was inoculated into CD4-positive cells, the vector was diluted 3-fold.

The mNDK HIV-1 vector was diluted to obtain one blasticidin-resistant cell colony in normal HeLa cells per a 10-cm culture dish.

After washing, peroxidase-conjugated anti-rabbit antibody (VECTOR) was diluted 1 5,000 in blocking buffer and 100 μl was added to each well.

After freeze-thawing of oocytes, FokI-dCas9 vector was diluted in DNase-free PBS at 5 ng/μl, and then microinjection was performed.

The pU6:: unc-119 sgRNA vector was diluted to 2 ng/µl and used as a template to generate two overlapping fragments.

Each vector was diluted in lactated Ringer solution such that 1 × 10 vector genomes (VG) were injected in 25 μl along the tibia into the TA and EDL muscles, as previously described by others [ 21].

Vectors were diluted and the LMH cells were prepared for the invasion assay.

For injections, AAV vectors were diluted in phosphate-buffered saline (PBS) to 1.2 × 10– 8.5 × 10 vectors genome/100  μL.