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We found that the genetically engineered MSC transduced by the lentiviral vector were able to secrete hNGFβ at physiologically relevant concentrations as measured by ELISA.
gas1Δ cells carrying the vector were able to grow in the presence of sorbitol, but not when the osmotic stabilizer was absent (Fig. 6A).
Control cells that were transfected with GFP-GalT or GFP empty vector were able to migrate into and cover more than half of the scratched area in 24 hours (Figure 3A, middle and right panels, respectively).
ZHY3 cells transformed with the empty pYES2 expression vector were able to grow on synthetically defined medium only when supplemented with Zn (750 μM to 2 mM).
Neuroblastoma cell lines, transfected ex vivo with the LID vector, were able to express high levels of IL-2 and IL-12, even if cotransfected with other cytokine genes.
Nevertheless, these effects appeared to be Notch-dependent, since MDA-MB-231 cells treated with two different Pin1 shRNAs and transduced with a N1-ICD-dPEST expressing vector were able to fully regain chemoresistance, as shown by recovery of M2FE in presence of the chemotherapeutic agent (Fig 4E and supplementary Fig S4A).
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This combination vector was able to suppress HIV-1 infection without impairing HIV-1-dependent effector activities of CD4ζ.
The most prominent advantage of this approach is that the selected output vector is able to improve the accuracy of reentry landing.
The ligand-directed vector was able to specifically deliver imaging and therapeutic transgenes to tumors in mice, rats, and dogs while sparing the normal organs.
The resulting vector was able to infect and to move in N. benthamiana plants in a manner similar to the wild-type GVA, but it was not stable and the inserted sequence was lost from the genome.
The vector was able to inhibit the expression of the HCV IRES in Huh-7 cells, with the inhibition level dependent on the shRNA74 to SLN ratio and on the shRNA74 dose added to the culture cells.
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