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At 72 h after transfection of the integrated donor and all-in-one CRISPR vector with or without MS2-CtIP LoADing vector, we observed a highly heterologous pattern of three kinds of fluorescence without any drug or fluorescence selection (Fig. 3b).

Moreover, using a NOX1 expressing vector we observed that NOX1 overexpression (thirteen-fold increase) is sufficient to increase endothelioma cell migration and tube-like structure formation (Figure S4).

In Podocalyxin-transfected cells, but not cells transfected with empty vector, we observed a striking increase in microvillus formation (figure 1A D).

In contrast to cells transfected with empty vector, we observed a reduction in the characteristic STAT1 1 SIE, STAT1 3 SIE, and STAT3 3 SIE complexes in the presence of H5N1 NS1 protein (Figure 2).

When we compared cells that were infected with a shRNA vector targeting p53 to cells infected with a control shRNA vector, we observed lower p53 and p21CIP1levels after γ-radiation.

Furthermore, after cotransfection of hClC-6 with a Rab4-GFP expression vector we observed a high degree of colocalization, analogous to overexpression in COS-1 and HeLa cells (data not shown).

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After transfection of the expressing vectors, we observed that wild-type IGFBP5 was mostly localized in the nucleolus.

As previously noted with a VSV-G-pseudotyped vector (14), we observed that the injection of two different Mokola-pseudotyped vectors co-infected the majority of astrocytes within the injected region (data not shown).

Compared to empty vector control, we observed a significant increase in basal ROS content in three independent ME2 shRNA knockdown cells (Figure 4B).

In contrast to cells transfected with vector alone, we observed an increase in SOCS1 but not SOCS3 protein in cells expressing H5N1 ns1 (Figure 5A).

One week following lentiviral vector injection we observed massive infiltration of CD45 positive cells in areas with remnants of GFP expression.

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