Sentence examples for vector was treated from inspiring English sources

All the features extracted were combined into a vector and the vector was treated as distinguishing features for splicing detection.

The HA encoding gene in the pCI vector was treated with Xho I, Klenow polymerase fragment, then subsequently digested with Sal I, and cloned into the pFastBac plasmid, a baculovirus (BV) transfer vector (Invitrogen).

To obtain in-frame fusions, the resulting vectors were cut with BglII and subsequently 1 µg of cut vector was treated with 5 U mung bean nuclease (New England Biolabs) for 1 hr at 30°C to remove overhangs and ligated.

Prior to ligation, the AvaI-digested vector was treated with alkaline phosphatase (Gibco BRL) following the manufacturer's recommendations for 5' overhangs.

ΔCt values for individual pools were calculated and the ΔCt average for the empty vector was treated as an arbitrary constant and used to calculate ΔΔCt values for all pools.

The resulting PCR products and the pET-Pfu vector was treated with the SacI- and BlpI-Fastdigest restriction enzymes (Fermentas) and subsequently fragments of the expected size were gel purified as described above.

There was no such interaction even when cells with the basic/empty vector were treated with HU.

When the linearized vector is treated with T4 DNA polymerase, in the presence of the absent base, long single-stranded overhangs are generated that are suitable for cloning.

In previous PST studies [1, 2], the surface normal vector and the error vector are treated as two entities and are solved independently.

(E) CGNs transfected with GFP vector plus wild type MCU, MCUD260A mutant, MCUE263A mutant or empty vector were treated with 70 μmol/L H2O2 for 24 h.

(A) Cells of the Atg31∆ strain expressing wild-type (WT) Atg31, the Atg31-Ser174 mutant (S174A), or a control vector were treated by nitrogen starvation (SD-N), rapamycin or amino acid starvation (SD-AA) and assessed for autophagy activity.