In addition, infectious titers of the LV-GFP vector were quantified by infection of 293T cells, in which the R0.8 promoter is active, followed by flow cytometry on a FACSCalibur (Becton Dickinson, Franklin Lakes, NJ, USA).
The proteins (Pro, P10, P8, RdRp, VPg and CP) expressed from pGAD T7 vector were quantified using HA polyclonal antibody as primary antibody, where as MP and the deletion mutants of MP expressed from pGBKT7 were quantified using cMyc monoclonal antibodies.
RNA content of lentiviral vectors was quantified with a one-step RT-qPCR that allows reverse transcription (RT) and amplification to take place in the same reaction tube.
Transfection efficiency and cellular internalization of the vectors were quantified in the presence of a panel of inhibitors of various endocytic pathways.
For this purpose, the uncertainty in the observed difference of the sample mean vectors is quantified by defining a confidence region which contains the true difference of the population mean vectors with a prescribed probability.
To provide a comprehensive evaluation, in our study the differences between the hyper-spectral feature vectors are quantified using classical metrics that were widely used in the development of hyper-spectral classification schemes.
Vectors were quantified using the forward primer 5'-GGCGGAGTTGTTACGACAT-3' and the reverse primer 5'-GGGACTTTCCTACTTGGCA-3' specific for the CMV promoter sequence.
To verify that inducing expression of the lysozyme variants in the chemostat cultures was taking place and producing the expected consequences, lysozyme transcripts originating from the induction vectors were quantified using qRT-PCR and secreted lysozyme activity determined using an enzymatic assay (Fig. 2a, b).
The vector genome copy number was quantified by real-time PCR using Taqman probe technology (Applied Biosystems, Carlsbad, CA, USA) and primers specific for the CMV promoter sequence.
First, whole vessels were incubated with Ad vectors and luciferase activity was quantified using an IVIS imaging system.
