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In the current study, this vector was produced using a combination of cis and trans phage display.
Using bacterial artificial chromosome (BAC) technology, a canine herpesvirus (CHV -based reCHV -basedvaccine vectorecombinantced for the devaccinent of an antifertility vectore for foxes.
In brief, AAV5 vector was produced by the 2-plasmid, co-transfection method.
Then, in several cloning steps using synthetic adaptors, primers and PCR reactions, the pTFBL-p3 vector was produced.
The rAAV2-UF5 vector was produced at the University of Florida Gene Therapy Center as described previously [86].
The pSico-Rac1 lentiviral vector was produced by digesting the pSico construct with HpaI-XhoI restriction enzymes (Promega), inserting shRNA oligonucleotides to target the mouse Rac1 gene and religating using T4 DNA ligase (Promega).
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Four types of transfer vector were produced: pTransVP3-S(N /full-length BMP-2, pTraN /full-lengthl-length BMP-2, pTransVP3-S/full-length BMP-2 and pTransH1/full-length BMP-2.
At this step, the vector is produced.
The final output feature vector is produced using a two-staged feature vector postprocessing procedure.
The final 1968-bit globinarynary vector is produced by concatenating the vectors obtained from the above two approaches.
Constructs expressed in the pLZ vector are produced with an N-terminal hexa-histidine tag composed of the amino acids MRGSHHHHHHEFGS.
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