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Targeting with the Col1a1-frt vector was monitored by Southern blotting of EcoRI digested DNA hybridized to the Col1a1 3′ probe, which is an 842 bp genomic PstI-XbaI fragment.
To exclude interference from the activity of other PTs (particularly the low affinity ones), the dynamics of Pi transport of cells transformed with either p112A1NE- CmPT1 or the empty vector was monitored at 0.5 mM Pi.
In all cases, the assembly of the prefabricated HAC vector was monitored by resolution of the individual species within the ligation reaction using Pulsed Field Gel Electrophoresis (PFGE) (Bio-rad, DR-III), or Field Inversion Gel Electrophoresis (FIGE) (Bio-rad) and confirmed to have proceeded efficiently as shown in Figure 2.
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The growth and metastasis of the tumors (HCT116 inserted with mCherry_luciferase vector) were monitored by bioluminescence imaging using the IVIS imaging system.
The growth curves of CNE2 cells stably transfected with TFPI-2 gene (CNE2- TFPI-2) or empty vector (CNE2-empty vector) were monitored by cell counting.
To examine the effects of Emuc in vitro using our viral vector transduction system, the morphology of the cultured adherent cells transduced with the viral vectors was monitored by microscopy.
The β-galactosidase activity of M. smegmatis harboring the vector pAZI9018b was monitored.
Upon transduction with a control (solid symbols) or a MYBL2 -specific (open symbols) shRNA-expressing vector growth was monitored for 6 days.
A FLAG-tagged plasmid of NRF2 was thus transfected into cells along with either SAP130 or empty vector and protein stability was monitored over time.
Mice were i.p. injected with the AAV8 vectors, and luciferase expression was monitored 2 weeks post infection (p.i .. AAV vectors containing mIP2 [ 15] exhibited potent, pancreas-restricted luciferase expression (Fig. 1b).
TIEG1−/− osteoclast precursors were infected at day 2 with either adenovirus expressing TIEG1 or vector alone and TIEG1 expression was monitored by QPCR at day 3 and 4 to determine relative TIEG1 expression levels.
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