Sentence examples for vector was mixed from inspiring English sources

For bottom-up in vitro SCRaMbLE, 200 ng acceptor vector was mixed with the donor fragments pool (1000 ng in total) in a reaction volume of 50 μl with 1 μl of high concentration Cre recombinase (NEB, M0298M).

Then, 20 µg pGCL vector was mixed with 15 µg pHelper1.0 vector (gag/pol component) and 10 µg pHelper2.0 vector (VSVG component), packed and transfected into 293T cells.

In co-transfection experiments, pCMV-Sp1 or pSG5-Ap1 expression vector was mixed with the luciferase vectors at 1 4 concentration ratio.

100 μl sterile water containing 20 μg/ml vector was mixed with 4 μl FuGENE HD transfection agent (Roche, Basel, Switzerland).

For the annealing process, 1 μl of T4 treated vector was mixed with 2 μl of T4 treated PCR product and allowed to incubate at 22°C for 1 hour. 1 μl of 25 mM EDTA pH 8.0 was then added and the reaction incubated for 5 mins.

200 ng of the linear vector was mixed with insert DNA at equal molar ratio in a 20 µl volume containing Phusion DNA polymerase reaction mixture (Finnzymes).

In the binomial crossover, the target vector is mixed with the mutated vector, using the following scheme, to yield the trial vector (u_i^G ).

The four pENTR vectors and a pDEST vector were mixed in the presence of LR clonase enzyme to generate the multi-integrase platform plasmid (Fig. 1B).

Around 20 ng of each entry clone and 80 ng of destination vector were mixed with 2 µl of LR clonase II Plus enzyme mix, and made up to 10 µl with TE buffer.

When cells reached about 70% confluence 95 μg of pVSVG, 68 μg of pREV, 176 μg pMDL, and 270 μg transgene containing lentiviral vector were mixed.

For ICAM-5 rescue experiments, plasmid p-EGFP-N1 and ICAM-5 constructs (PEF-BOS-ICAM-5, PEF-BOS-ICAM-5 Δcp or the empty vector) were mixed at 1 10 ratio and co-transfected into neurons.