Sentence examples for vector was maintained from inspiring English sources

The autonomously replicating shuttle vector was maintained at 25 to 115 copies per chromosome.

FISH analysis showed that the iHAC vector was maintained independently from the host chromosomes in CHO/iHAC/X53.

FISH analysis showed that the HAC vector was maintained independently from the host chromosomes in CHO/iHAC/X53.

Because the shuttle vector derived from this plasmid competed with the native plasmid it was derived from, the shuttle vector was maintained in low copy and was readily lost without selection.

A CHO-K1 cell line stably transfected with rat KV2.1 in a tetracycline-inducible vector was maintained in Ham's F12 media containing 10% fetal bovine serum, 1% penicillin-streptomycin, 1 μg/mL blasticidin, and 25 μg/mL zeocin at 37 °C in a 5% CO2 atmosphere.

HCT116 clones transfected with MUC1, MUC1*1110, or empty vector were maintained in DMEM with G418 and 0.5% FBS for 48 hours.

PRL-HeLa cells transiently transfected with a GFP-ER expression vector were maintained for 48 hours in a hormone-free medium, and were then treated with 10 nM E2, 100 ng/ml EGF or 10 nM 4HT.

CHO cells carrying the HAC vector were maintained at 37°C in Ham's F-12 nutrient mixture (Invitrogen) supplemented with 10% fetal bovine serum (FBS; JRH Biosciences, Lenexa, KS, USA).

Efficient regulated gene expression is also observed in vivo, where the vector is maintained long-term.

These Scp-21 cells expressing Fra-1 or control vector were maintained in DMEM supplemented with 10% fetal bovine serum and 5 μg/mL puromycin.

Expression vectors were maintained at constant total amounts via the addition of empty vectors.