Sentence examples for vector was inoculated from inspiring English sources

The E. coli (BL21 DE3) containing ptanET 28a vector was inoculated in LB broth with kanamycin and grown overnight in a 37 °C incubator shaker.

Therefore, when the ROD/B vector was inoculated into CD4-positive cells, the vector was diluted 3-fold.

Therefore, when the HIV-1 vector was inoculated into CD4-positive cells, the vector solution was diluted 20-fold to normalize the transduction titer.

To modify the EZ-Tn5-carrying plasmid pMOD-2 (Epicentre) for use in C. perfringens, a single colony of an E. coli strain encoding the EZ-Tn5 pMOD-2 vector was inoculated into 10 ml of LB broth supplemented with ampicillin (LBA) and then incubated overnight at 37°C with shaking (250 RPM).

The ALSV vector was inoculated into G. hybrida 'Polarno White' plants overexpressing the Arabidopsis FLOWERING LOCUS T (AtFT) gene.

A single colony of A. tumefaciens harboring binary vector was inoculated into LB medium supplemented with 50 μg/mL kanamycin and cultivated with shaking of 250 rpm at 28 °C for 12 16 h to an optical density of 0.5 1.0 at 600 nm.

(G) Human gastric cancer cell line SGC7901 cells stably overexpressing SENP3 or vector were inoculated into nude mice to form tumor xenografts, and the tumors were then recovered, minced into small pieces and implant beneath the serosa of the stomach of other mice to grow for eight weeks (upper).

BGC823 cells stably infected with HPIP shRNA lentivirus or empty vector were inoculated s.c. in the dorsal skin fold of each nude mouse.

To explore whether the level of SPRY4-IT1 expression could affect tumorigenesis, SPC-A1 cells stably transfected with pCDNA-SPRY4-IT1 or empty vector were inoculated into female nude mice.

To further explore whether the inhibition of EZH2 expression could affect tumorigenesis, SPC-A1 cells stably transfected with sh-EZH2 or empty vector were inoculated into female nude mice.

For experimental metastasis, OCUM-2MD3 cells expressing Wp85, Δp85, or empty vector were inoculated intraperitoneally into 4-week-old female athymic nude mice (NCr- nu/nu), obtained from Charles River Laboratories (Portage, MI, USA).