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The vector was injected via an intravenous tail-vein injection with specified genome copies (gc)/mouse.
This cocktail, called a vector, was injected under the skin of the arm.
Also, an effective tumor mass reduction was observed when the vector was injected directly into C3 tumors implanted in C57BL/6 mice.
In a randomized double-blind placebo-controlled phase I clinical study in DMD boys, AAV2.5 vector was injected into the bicep muscle in one arm, with saline control in the contralateral arm.
Furthermore, a mammalian CDR1as circRNA over-expression vector was injected into zebrafish embryos with the genetic background of normal expression of conserved miR-7 but depletion of the CDR1 gene locus in the embryonic brain (Kapsimali et al., 2007).
The Tg vector was injected to fertilized eggs to generate transgenic mice (Fig. 1A).
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HCT116 cells or FANCM-knockout HCT116 cells expressing Rad52 shRNA or the control vector were injected subcutaneously into 6-week-old female BALB/c-nu mice (n = 5 per group).
CFP-expressing Met-1 cells prepared by retrovirus infection of a CMV promoter-CFP vector were injected i.v. into the tail vein of each mouse.
1×106 Hcells cellstablyly expressing pIRES2-SBP1 or pIRES2 vector were injected subcutaneously into the flank of NIH-III nude mice (NIH-Lyst bgFoxn1nuBtkxid) (Charles River Laboratory, NY) (5 mice per group).
SGC7901 cells (1 × 10) with Dox-inducible CXXC4 expression vector were injected subcutaneously into the flank of nude mice.
Stable cell lines carrying either OCT1 expression vector or the control vector were injected subcutaneously to the right flank of nude mice to allow xenograft tumour formation.
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