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To do so, the vector was first digested with SpeI, blunt ended with T4 DNA polymerase and religated.
Briefly, a ∼9.8 kb region used to construct the targeting vector was first sub cloned from a positively identified BAC clone using a homologous recombination-based technique.
To generate a lentiviral vector for T cell transduction, a pEF1-pcs-puro vector was first constructed based on a HIV-based vector pHxEGFPWP [51].
In central Africa, this spreading vector was first collected in 2000 in Cameroon, in 2003 in Equatorial Guinea, and in 2006 in Gabon [15].
A SB transposon-based gene-trap tTA vector was first designed for and tested in zebrafish embryos [ 13].
Briefly, the psicheck-2 vector was first digested with Not1/ Xho1, purified on a 1% agarose gel and extracted using QIAquick Gel Extraction Kit (Qiagen, Venlo, The Netherlands).
Similar(43)
In a SVQ, the input vector is first split into multiple subvectors [7], and then the resulting subvectors are quantized independently [8, 9].
Given a point on a curve, the orientation of its tangent vector is first estimated and then curvature is calculated by Gaussian differential filtering.
This signal vector is first multiplied by an M × n k precoding matrix T k and then transmitted through M transmit antennas.
In the t th optimal atom selection for y i, the following vector is first defined: y i, j ( t ) = D i ( t - 1 ) d j x i ( t ) x j = D i, j ( t ) x i, j ( t ) ( j = 1, 2, …, K ), (10).
Bartonella quintana, a pathogen that is restricted to human hosts and louse vectors, was first characterized as the agent of trench fever.
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