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Exact(19)
If needed, empty expression vector was added to the reaction to equal the total amount of plasmid in all transfections.
Plates were again washed 6×with PBS/0.05% Tween 20, then 100 µl of 3-amino-9-ethylcarbazole substrate (Vectastain AEC substrate kit; Vector) was added.
Excised colon was scratched in PBS (containing 1.5 mM Ca2+) with a 30-gauge syringe needle, and, 3 min later, FITC-UEA (10 µg/ml, 10 min, Vector) was added.
Vector was added to the cells in their corresponding medium supplemented with 1% FCS.
Non-coding vector was added to keep the total DNA constant (4.5 7.5 μg).
For each experiment, a negative control (SKOV-3 cells transfected with empty vector) was added.
Similar(41)
This vector is added to the observation z i.
A reciprocal lattice vector is added after this process, possibly leading to a large-angle scattering [15 17].
Twenty-three acids acids encoded by the vector were added at the N-terminal of wild-type enzyme but no potential secretion signal peptides were predicted in wild-type or recombinant TlXyl43 enzyme.
This algorithm produces a set of locally optimal indices, since at each step, the best vector is added to the existing subspace (but obviously, it is not globally optimal due to its greedy process).
Two candidate solutions are selected randomly from the population and the vector difference between them is calculated and its weighted value is calculated by multiplying factor called mutation (0 1) and resulting weighted vector is added with the third randomly selected candidate solution which needs to be selected from the population other than earlier selected two candidate solutions.
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