Sentence examples for vector using from inspiring English sources

The amplified DNA was cloned into pET29a vector using NdeI and EcoRI.

To describe each vector using the least possible amount of information.

A pair of annealed oligonucleotides is assembled into the all-in-one vector using the Golden Gate assembly method.

MDBK cells were transiently transfected with this anti-B2M CRISPR/Cas9 vector using the Neon® transfection system (Invitrogen).

PCR products were introduced into the piggyBac vector using Gibson Assembly (GA) cloning30 with two GA mastermixes made in-house.

Sridhar, S. et al. Single-dose protection against Plasmodium berghei by a simian adenovirus vector using a human cytomegalovirus promoter containing intron A.

For the middle entry clone, Cre-ER T2 cDNA amplified by PCR was cloned into pENTR-TOPO vector using the Invitrogen TOPO TA cloning kit.

Briefly, the pGL3-TLR4-wt and pGL3-TLR4-mut plasmids were separately co-transfected with the pRL-TK renilla vector using Lipofectamine 3000 (Invitrogen).

4T1 cells were transfected with 1 μg of pCMV6_Zeb1 (OriGene) or empty vector using Lipofectamine 3000 (Invitrogen) according to manufacturer's instruction.

Initially, the cells were transfected with the pcDNA3 ECFP-DEVD EYFP ECFP-DEVD EYFPtor using lipofexpressionTX (Invectoren, #15338-100) as per the manusingurer's protocolipofectamine

A375 cells were transfected with pcDNA3-STAT3C plasmid or empty vector using Lipofectamine 2000 (Life Technologies, Inc., Rockville, MD) following the manufacturer's instructions40.