Sentence examples for vector treated with from inspiring English sources
(H) Scatterplots with boxplots show the total dendritic length distribution of neurons transfected with control vector treated with vehicle (Ctrl, n = 53, gray), with 50 nmol/L of TMRM (n = 31, blue) or 50 nmol/L of TMRE (n = 63, red), or transfected with BRAF treated without (n = 24, green) or with 50 nmol/L of TMRE (n = 32, purple).
After the second round of PCR the products are mixed with the vector, treated with UDG and allowed to anneal.
As described above the respective forward and reverse products were mixed with vector, treated with UDG and allowed to anneal.
The isolated fragment was inserted into pEU-E01 SspI- vector treated with the same restrction endonucleases.
For gene array analyses, total RNA was isolated from RAW264.7 control cells (empty vector) treated with rapamycin or cells expressing LAP, using the RNeasy mini kit (Qiagen).
The resulting DNA fragment was treated with XbaI and KpnI restrictases and ligated with pBluescriptII-SK vector treated with the same enzymes.
The generated complementary overhangs allowed introduction of the target gene into the vectors treated with T4 DNA polymerase using a simple annealing step, without use of any other enzyme.
(b) Fold change in mRNA expression of oligodendrogenic regulatory genes in NSCs transfected with dnGR or GFP (control) vectors, or no vector, and treated with cort, relative to vehicle-treated controls; n3.
Cells were transduced with a doxycycline-inducible RBM4 vector or control vector and treated with 100 ng/ml Dox for 24 hr.
(E) CGNs transfected with GFP vector plus wild type MCU, MCUD260A mutant, MCUE263A mutant or empty vector were treated with 70 μmol/L H2O2 for 24 h.
To obtain in-frame fusions, the resulting vectors were cut with BglII and subsequently 1 µg of cut vector was treated with 5 U mung bean nuclease (New England Biolabs) for 1 hr at 30°C to remove overhangs and ligated.
