Sentence examples for vector to yield from inspiring English sources

The fragments were cloned into pET21b vector to yield pET21b-Sdo1p constructs with C-terminal 6× -His-tag for purification.

Then, PhaB was cloned into the BamH I/Sal I sites between the TEF1 promoter and the CYC1 terminator of the same vector to yield pSP-GM2-phaAB. PhaC was cloned into the MCS of pSP-GM2 vector the TEF1 promoter and the CYC1 terminator.

Gel-purified and BamHI and KpnI digested CYP71AV1 gene was ligated into this vector, to yield 35S-CYP71AVI.

The inserted fragment was then recombined between the attR1 and attR2 sites in the multi-cloning site of the modified pUAST vector to yield pUAST-tw.

To express the core-coding region in eukaryotic cells, the NheI-XbaI fragment from these plasmids was cloned into the pCI eukaryotic expression vector to yield pHPI-8161 (HCV1and) and pHPI-8162 (HCV1a p34).

The udg ORF was subsequently positioned under the control of the CMV IE-chicken ß-Actin promoter/enhancer element in the pCAN expression vector to yield pCANudg. DF-1-derived cell lines that constitutively express UDGMVA were generated by calcium phosphate-mediated transfection of the udg-expression plasmid pCANudg into DF-1 cells followed by clonal selection of G418R cells.

The objective function took advantage of the number of support vectors to yield models that are more capable of generalization and are less computationally expensive.

The HIV-1 p17/p247/p24 and gag genes were cloned into these vectors to yield 12 different constructs (Table 2).

Plasmid DNA from entry clones was then used for LR clonase-mediated in vitro DNA recombination with appropriate destination vectors to yield FLAG and GFP epitope tagged constructs.

We calculated the angle between these vectors to yield a value Δ θ(t) for each time-point t in a single tube path.

This yields a training vector of 400 dimensions.