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Mutant phage that did not circularize miniPLE were sequenced, and, since mutants were not clean deletions of individual gene products, the individual gene products that were missing or mutated were cloned into an inducible vector to test for miniPLE circularization.
In the present study, a series of primers were designed in the 2000 nt upstream regions of porcine miR-181 and miR-1 and then the sequences were cloned into pGL3-basic vector to test their transcriptional activity.
The second stage is based on feature extraction and selection from the MCs located in the first stage; in the final part of this latter stage, these features are used as an input vector to test two classifiers based on a GRNN and support vector machine (SVM).
We have performed additional studies in 4 week-old GM1 mice injected bilaterally in the thalamus and DCN with the same dose of AAV2/1-CBA-mβgal vector used in this study (with and without i.v. infusion of liver-specific AAV vector) to test this possibility, but did not observe any improvement in motor performance compared to age matched untreated GM1 mice (data not shown).
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Further, three of the predicted peptides were cloned into protein expression vectors to test the biological activity.
To answer this question we randomly inserted a gene into a viable network and developed stable state vectors to test whether stability was retained.
The reporter vectors to test NICD/RBPJκ activity (wild-type and mutated versions, p4xwtCBF1Luc and p4xmtCBF1Luc respectively) were kindly donated by Professor Hayward.
For each of 26 = 64 system parameter combinations, there are thus 28 = 256 different initial value vectors to test for multi-stationarity.
Since CGP77675 blocks the activity of multiple Src family members [ 38], we used MEFs from c-Src knockout mice that were transfected with a c-Src-expressing vector or a control vector to directly test the role of c-Src.
A Spearman rank correlation test [ 26] was performed on each GO term vector and the upstream alignment score vector, in order to test whether each GO term is associated with high or low proximal upstream conservation.
A luciferase reporter assay with a psiCheck2 vector was used to test the specificity of the sponge sequences for miR-17 family miRNA binding.
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