Sentence examples for vector to remove from inspiring English sources

We subcloned the t-Darpp cDNA into the pcDNA3.0/Neo vector to remove a FLAG-tag present at the N-terminus of the received cDNA and restore its native sequence.

Considering the antibiotic marker introduced by the ClosTron vector pMTL007 (GenBank: EF525477), we modified the vector to remove the RAM (Retrotransposition-Activated selectable Marker) [40] in order to construct a marker-free mutant.

Vectors were low pass filtered at 10 Hz, and 10 data points were trimmed from the end of each vector to remove artefacts associated with the filter.

pcDNA3GST-Y354F(Δ1050–1189) pcDNA3GST-Y354F Δ1050 1189tion of the pcDNA3GST-Y354F vector to remove the 1050–1189 amino acid fragment.

When annealed oligonucleotides are inserted in these vectors to remove the ccdB gene, these pSHIN-G and pREFLECT-R derivatives then allow bacterial growth.

In addition, a systematic analysis of the transfer vector sequence was undertaken to minimise the amount of HIV-1 sequence within the vector and to remove signals that adversely impact on the processing of the viral genomic RNA (gRNA).

However, as model A achieves the highest overall specificity of 99.4% at the 95% confidence limit from spectra that have only been vector normalised (to remove baseline shifts), model A can be used to indicate which peaks are responsible for discrimination.

Moreover, the polar histogram and its post-processing procedures will be used to indicate the position and length of feature vectors and to remove noises as well.

The targeting vector was designed to remove the 1st to the 9th exon of Ace3 (which corresponds to the 1st to the 8th exon of previous Ace3) (Figure 3A) and was electroporated into 129Sv ES cells after linearization.

The vector was modified to remove a weak σ70 binding site present 24 bp upstream of the GFP open reading frame (two point mutations, A → G and T → G, were introduced, changing the putative σ70 binding site from TAGATT to TGGATG, with the consensus σ70 binding site being TATAAT).

Finally, the modified pCi vector was digested with NcoI to remove the vector fragment remaining between the promoter and the ADA cDNA.