Sentence examples for vector to prepare from inspiring English sources

Afterwards, the biopolymer attains a high cell density due to recruitment of specific stem cell populations can be used as a vector to prepare cell cultures.

In our previous study [29], we used the pcDNA3.1 expression vector to prepare plasmids encoding soluble CD40L with varying degrees of valency by fusing the extracellular domain (ECD) of CD40L with multimerization scaffolds selected from other proteins.

We first used pPCR-XL-TOPO plasmid vector to prepare a cDNA library using total RNA purified from the whole guts of fifth-instar larvae of ECB.

Therefore, we used lambda Uni-ZAP RX vector to prepare a second cDNA library using mRNA purified from the guts of fifth-instar larvae of ECB.

This DNA fragment was utilized to replace the PTD-J fragment which is franked by NdeI and EcoRI sites of the pET22b-PTD1J1-DsRed pET22b-PTD1J1-DsRed pET22b-PTD1J1-DsRed

As such, there is an urgent need to improve our knowledge of the behavior, ecology, and genetics of this somewhat-understudied vector to prepare for future vector control strategies.

The shuttle vector was used to prepare replication deficient adenovirus using the AdEasy system (Stratagene) as previously described [14], [15].

Transposons were released from the vector and used to prepare transposomes for electroporation of rickettsiae according to the manufacturer's suggested protocol.

Agrobacterium tumefaciens strain C58C1, transformed with the pCAMBIA1300 binary vector, was used to prepare the T-DNA-tagged lines.

The ABI PRISM BigDye cycle-sequencing kit (Perkin Elmer, Foster City, CA), and specific primers, or the primers for the M13 forward and reverse priming sites of the pCR2.1 vector, were used to prepare the sequencing reactions.

Briefly, 24 40  μg of total plasmid DNA (luciferase reporter gene vectors) were used to prepare a DNA-transfection reagent mixture with Opti-MEM reduced serum medium (Life Technologies), and 60  μL Lipofectamine 2000.