Sentence examples for vector to make from inspiring English sources
The DNA of any gene of interest from a donor organism (such as a human) can be cut out of a chromosome and inserted into a vector to make recombinant DNA, which can then be amplified and manipulated, studied, or used to modify the genomes of other organisms by transgenesis.
The instrument requires pointing along the spacecraft's ram velocity vector to make this measurement, thus requiring stabilization in pitch and yaw.
While only permeability was included in the present study, the technique can easily be extended to include other parameters in the search vector to make it a general tool for more complex reservoirs.
The PCR products were purified and used in the following BP Cloning reaction, which used pDONR-221 vector to make Entry Clones of synthetic genes of hCRs.
The N-terminal V-5 tag sequence and HpaI site were introduced in the pLew82T7 vector to make pLew82T7 N-V5.
The coding region of Als5p between a 5' NotI site and a 3'XhoI site was generated by PCR and was ligated to the modified vector to make pJL1.
A set of necessary conditions for the choice of diffusion gradient vectors to make the linear equations nonsingular for the estimation of the diffusion matrix are given in a coordinate free manner.
The following were added to the transient expression vectors to make 21 transformation vectors: T-DNA border LB and RB, selection marker gene HptII (for hygromycin) or NptII (for G418/kanamycin) under the control of the 35S promoter, and the sequence ori-REP-STA from pVS1 for plasmid stabilization and replication in A. tumefaciens.
The full-length cDNA and five fragments of AtBPC6 were cloned in ENTRY-vectors and recombined into binary expression vectors to make GFP-fusion proteins.
We have also added a small pseudo-count to each entry of the vectors to make sure that every entry is non-zero.
After sequence control, all genes were cloned (in pARC113) and/or recombined (in pARC064, pARC258, and pARC259) in the appropriate vectors to make the fusion constructs.
