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A vaccine vector with low host reactivity, such as observed with PEPV or FeP2, might have the advantage of being a good vector to include adjuvant genes to enhance immunogenicity.
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Galloway et al. recently described a method to alter vectors to include Type IIS restriction enzymes for high efficiency cloning.
The expansion of R. felis hosts and potential vectors to include mites, lice, and ticks (both Ixodid and Argasid) further highlights the infancy of the field.
The p201N vector is a p201BK [ 37] vector modified to include an nptII selectable marker cassette and I- SceI and I- PpoI restriction sites (Additional file 6).
To establish a vector system in which convergent promoters drive the expression of short complementary RNAs, we modified the pLXSN retroviral vector (Clontech) to include convergent human U6 and H1 RNA polymerase III promoters.
The C 50 estimate is employed to select different acoustic models (back-end approach) which are trained on feature vectors appended to include the C 50 value (front-end approach).
This study extends the prospective safety testing of recombinant type 5 adenoviral vectors specifically to include Ad5-seronegative volunteers, and provides evidence that in this group the 1010 PU dose is of comparable immunogenicity to, and better tolerated than, the 1011 PU dose.
piggyBac transposons are favored over other elements as they are able to transpose large DNA fragments (9.6-14 9.6-1420] makbng them suitable for applications in dual expression vectors designed to include selection markers, transcriptional activators or immune enhancer elements.
While luciferase expression and bioluminescence imaging cannot be used in humans, our data provide proof-of-concept that CRAd vectors modified to include high-sensitivity imaging systems like the radionuclide-based somatostatin receptor (SSTR) or human sodium iodide symporter (hNIS) transgenes may improve the outcome of human gene therapy protocols.
The pCa4B vector was modified to include the following features from the pHStinger vector [ 53]: the pHStinger polylinker (replacing the pCa4B polylinker), a minimal Heat shock protein 70 (Hspromotermoter driving a GAL4 or GFP reporter gene, and gypsy chromatin insulators to block influence of flanking enhancers that would otherwise modify reporter expression via enhancer trap effects.
This vector was design to include six repeats of Myc-tag at N-terminus and two repeats of S-tag at C-terminus of full-length Shh protein.
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