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For in vitro pull-down assay, the complementary DNAs (cDNAs) encoding TT2, TTG1, and TTG1 mutated forms were cloned into pMAL-c2x vector to generate MBP-tagged proteins.
Fragments from each end of the NbSKRP gene were cloned into the pTRV2 vector to generate silencing constructs TRV NbSKRP-5′ and TRV NbSKRP-5′.
The amplicon was cloned into TA vector to generate the recombinant plasmid pHR1.
The purified fragment was then cloned into the pGEM-T easy vector to generate pGEM scFv construct.
The replica LOS signal is then multiplied by the LOS array steering vector to generate multi-antenna signals.
The amplified cellulase cDNA products were cloned into pGEM-T vector to generate plasmids pGEM-2AeglII and pGEM-cbhII.
It was inserted into BstBI site of PLVUT-1 vector to generate PLVUT-GFP vector.
The PCR products amplified by primers (BDF3/BDR3, Table 2) inserted into pcDNA3.1 vector to generate pcDNA3.1-grouper β-defensin.
The four entry clones were recombined with a destination vector to generate the multi-integrase platform plasmid.
The 3'UTR of PPARγ was inserted into a luciferase expression vector to generate a luciferase reporter construct.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com