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Additionally, we also used the empty pMyNT vector, to check for GroEL1 binding in the absence of a his-tagged protein.
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All sequences were searched for vector segments to check for insert integrity.
HEK 293 cells transfected with empty pEGFP-N3 vector were used to check the localization of GFP fusion proteins.
Recombinational cloning was useful in this study where the same set of ORFs could be cloned into a large set of different expression vectors without the requirement to check for compatible restriction sites in each vector or their absence within the ORFs.
The pSK vector control was used to check for any nonspecific responses, and no specific bands were detected with either northern blotting or reverse transcription polymerase chain reaction (RT-PCR).
These two vectors were also used to check where gene transfer was accomplished by Agrobacterium on cotyledonary node explants monitoring (transient) gene expression of GUS and DsRed, two days after cocultivation.
Again, the advantage of potentially less preprocessing than the two-vector method is erased by the need to check if a vector has been processed.
In addition a support vector machine approach was used to check the final product quality.
From the expression in the judgment of a candidate section, only the matrix that contributes to global motion ({varvec{{W}}}_{{A}text {2}}^) is needed to check the vector closure condition.
To more precisely evaluate whether the observed suppressive effects were specific for the target sequences, we used the psiCHECK-2 vector, which is typically used to check the knockdown efficiency of a target sequence by siRNA or miRNA [ 22].
As discussed earlier, the extra steps to check if a vector or a pair of vectors have already been processed takes longer than the benefit of potentially fewer steps needed for preprocessing.
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