Sentence examples for vector the expression from inspiring English sources

We observed that compared to cells transfected with the empty vector, the expression level of PTEN and SALL1 in SALL4A-expressed cells was decreased by 30% and 35%, respectively.

Twenty-four hours following transduction of human melanoma cell lines with the SOCS1 and SOCS3-expressing retroviral constructs or empty PINCO vector, the expression of EGFP was confirmed in each cell line by flow cytometry.

Regarding the variables of mechanical and electrical fields as the elements of the state vector, the expression of the localization factors in disordered periodic layered piezoelectric composite structures is derived.

RCC4-EV cells lack VHL activity and constitutively express HIF-1α and HIF-2α proteins, whereas in the RCC4-VHL subclone, which is stably transfected with a VHL expression vector, the expression of HIF-1α and HIF-2α proteins are induced only under hypoxic culture conditions20, 25.

To further confirm that the effects of miR-495 are mediated by repression of JAM-A in breast cancer cells, knockdown of JAM-A in MCF-7 cells by miR-495 mimic was restored by transfecting JAM-A cDNA vector, the expression of which is not regulated by miR-495 due to its lack of the 3′-UTR (Fig. 5A).

In order to realize a selection-free PCRRC function in the designed vector, the expression cassette of lysis gene E of bacteriophage Phi-X174 as suicide gene was introduced into pOmni between the 6His tag region and BGH Poly(A) site (Fig. 2).

The most commonly used SFV expression system is based on two plasmid vectors: the expression vector contains the SFV non-structural and the foreign genes and the helper vector harbors the SFV structural genes.

In both vectors the expression of SSO genes was maintained under the endogenous SSO1 and SSO2 promoters, respectively.

From these entry vectors, the expression cassettes were eventually transferred into the adenoviral vector backbones via site-specific recombination.

Our study demonstrates the importance of cis-acting elements to enhancing the transduction ability of lentiviral vectors and the expression of vector transgenes.

Cloning into a pGEX vector allowed the expression as GST fusion proteins.