Sentence examples for vector that lacks from inspiring English sources

Sequences that function as ARSs in yeast occur on average every 20 to 40 kb in all the eukaryotic genomes thus far examined [ 14, 15], which suggests that most mammalian chromosomal regions can be readily isolated by TAR-cloning with a vector that lacks an ARS.

The reduction in the ZIC3 nuclear localization ratio was not markedly reversed by the cotransfection of a Kpna1 or Kpna6 expression vector that contains the siRNA target sequence but was reversed by a modified Kpna expression vector that lacks the siRNA target sequence (ΔsiRNAseq).

SMN levels were elevated nearly three fold compared to the pM13 vector and a pMU3 vector that lacks the promoter that drives the In711 RNA (pMU3KO) (Figure 4A).

These resulting fragments containing the homologous recombination regions and the hexon genes were purified, then recombined with a SwaI-digested Ad5 backbone vector that lacks the hexon gene, pAd5/ΔH5 [26].

For SeV vectors, we used a non-transmissible vector that lacks the F protein.

Cotransfection of these two vectors into HEK293 cells decreased luciferase expression to a greater extent than a control vector that lacked miRNAs (Fig. 2B).

In contrast, the parental vector that lacked the ASO, pM13, did not result in significant levels of SMN induction (Figure 7C, 7D).

The constitutive promoters were cloned into lentiviral self-inactivating vectors that lack endogenous promoter activity from the long terminal repeats.

Engineering adenovirus vectors has taken the direction of deleting larger and larger segments of the viral genome, leading to high-capacity 'gutted' vectors that lack all viral coding sequences [ 14].

We previously demonstrated that Alu-containing TAR cloning vectors that lack ARS elements are efficient for selective isolation of human DNA from human-rodent monochromosomal hybrid cell lines [ 27].

As seen in Table 1, the majority of genomic fragments surveyed support de novo HAC formation at frequencies consistent with previous reports using vectors that lack genomic fragments or contain a limited number of other genomic fragments [ 3, 4, 16, 34].