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The human proinsulin gene cDNA engineered with concensus furin cleavage sequences was inserted into an advanced lentiviral vector that contained CMV early promoter.
The vector that contained the corresponding gene harboured the strong constitutive glycolytic promoter from glyceraldehyde-3-phosphate dehydrogenase (gpdA) of A. nidulans.
The cDNAs of Ku80 and Ku70 were subcloned into a pcDNA3.1 expression vector that contained a V5-tag in the C-terminus of the cDNA.
We constructed the Tg vector that contained the promoter of a house keeping gene, elongation-factor 1α (EF1α) and GFP-tagged NRG1β1 cDNA.
E. coli plasmid pCR2.1TOPO (Invitrogen) was used to construct a suicide vector that contained a 4.0-kb sequence from S. coelicolor spanning the melD operon, in which the melD operon was replaced by the aac 3(IV -oriT cassette [52] (FIV -oriT
To improve the transduction efficiency in mammalian cells, the c-Photina gene was optimized for mammalian codon usage (as described for Photina® in [6], and then fused to a mitochondrial tag (human Cytochrome C Oxidase, subunit VIII) [14], in an expression vector that contained no antibiotic resistance gene.
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The pCYH10 vector is a promoter-less vector that contains a complete coding sequence of GUS.
(ii) is the vector that contains the mean energy of the 88 piano notes.
SeD on receiving the request creates the performance estimation vector that contains the performance estimation values.
E is a negative control vector that contains green fluorescent protein (GFP) cassette.
(i) is the vector that contains all the possibly played notes.
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