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Repeated passaging of LV.FLPe.PurR-transduced human myoblasts in the presence of puromycin revealed no apparent differences in the growth rate between cell cultures exposed to the different amounts of FLPe-encoding vectestedsted (not shown).
Finally, we visualized the selective transcriptional targeting of the most promising vector tested in this study, AdCXCR4, in breast cancer tissue versus normal breast tissue (Fig. 6).
To date, only ten patients have been treated with the self-complementary vector tested by Nathwani et al, 6 and as such, many large cohorts remain to be tested.
48 hours after transfection, luciferase assays were performed and showed that only KLF15 was able to reduce the minimal promoter activity of T1 transcript by about 50%, at each concentration of vector tested.
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Thus, in vivo gene expression in genetically engineered neural progenitor cells is temporally limited and mostly restricted to undifferentiated NPC using the viral vectors tested.
Upon nasal instillation in mice of complexes made with various glycosylated PEIs, a high luciferase activity was observed while the green fluorescent protein (GFP) expression was similar for all the vectors tested with few cells expressing GFP.
Of those vectors tested, recombinant adenovirus (rAd5) was the most immunogenic, inducing the highest titer anti-B5 antibodies and conferring protection from sublethal vaccinia virus challenge in mice after a single immunization.
The various combinations of Entry to Destination vectors tested are indicated in Table 3.
Among the AAV vectors tested, rAAV1 and rAAV2 displayed the most promising results when used for RI.
The AAV2/6 vectors tested so far in this study do not exclusively target Müller glial cells, but display tropism for other neural components of the inner retina (bipolar, amacrine, horizontal and ganglion cells).
Almost half of the vectors tested positive for more than one vertebrate species, T. cruzi infection was detected in 42% of the specimens and there was a significant difference in T. cruzi infection between domestic and peri-domestic habitats.
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