Sentence examples for vector template from inspiring English sources

A new control approach named Unit Vector Template Generation (UVTG) is designed for SAPF and presented in this paper.

Each PCR primer consisted of an ∼20-nucleotide (nt) sequence that annealed to the vector template and an ∼30-nt overhang that overlapped with one end of the target DNA.

The whole cassette is PCR amplified from a vector template (Table 1) with specific oligonucleotides that add ∼50 bp of sequence homologous to each side of the desired site of insertion in the gene of choice.

After PCR, these products were pooled, digested with Dpn1 for 4 hr at 37°C to remove vector template, and concentrated by isopropanol precipitation.

IRES-Emerald GFP (eGFP) cDNA was generated by PCR from an existing vector template (pHR-IRES-eGFP [ 19]) and cloned into the EcoRI/BclI sites of pOPINEE12G.

This was determined from the vector template using standard dye-labelled primer protocols from Applied Biosystems and a model 373A automated DNA sequencing system (Applied Biosystems, Inc., CA, USA).

These vector templates were used for generation of CMG mutants through PCR based mutagenesis.

YFP D6PK and kinase-dead YFP D6PKin were amplified in a similar manner as described for PIN1 and PIN3 from previously described vector templates (Zourelidou et al., 2009) and inserted into the EcoRV site of pOO2.

In Sections 4 and 5 we define two additional problem templates: the Outside Vector Multiplication Template, and the Multiple String Vector Multiplication Template, and describe modifications to the algorithm of Valiant which allow to solve problems that sustain these templates.

Beginning with an estimate from image realizations of the optimal generic (nonincreasing) window function, the algorithm switches (exchanges) a set of observation vectors (templates) between the optimal function's kernel and the kernel's complement.

In Section 3 we describe the Inside Vector Multiplication Template - a template which extracts features for problems to which Valiant's algorithm can be applied.